Preparation of biodiesel from crude oil of pongamia. Biodiesel production from pongamia pinnata oil using. The micropropagation of chestnut via axillary shoots involves four stages. Sep 10, 2014 aseptic method of clonal propagation is called as micropropagation and it offer the advantage of large number of truetotype plantlets can be produced with relatively short time and space from a single individual. Department of biotechnology, gulbarga university, gulbarga585106, karnataka, india corresponding author.
Abstract pongamia pinnata is a legume plant which has great. This stage begins with the excision of meristem tissue from an identified stock plant. The micropropagation of chestnut from tissues taken from adult trees appears usually to be feasible when these tissues retain physiologically juvenile characteristics, as is the case of basal shoots and stump sprouts vieitez et al. Micropropagation is the truetotype propagation of a selected genotype using in vitro culture techniques. The yield of biodiesel obtained was 97 per cent by using oilmethanol molar ratio 12. Principles of tissue cult ure and micropropagation.
The present study deals with the transesterification of pongamia pinnata oil by means of methanol to study the feasibility of methanolysis process by using potassium hydroxide catalysts. Micropropagation in horticulture, by s gunn the plantsman new series vol. Names of pongamia pinnata in various languages of the world are also given. Pongamia pinnata has recently been recognized as a viable source of seedoil for production of biofuel to meet the demand of the burgeoning. After tranesterification of crude oil shows excellent properties like calorific value, iodine number, cetane number and acid value etc. Millettia pinnata is a species of tree in the pea family, fabaceae, native to eastern and tropical asia, australia and pacific islands. The potato micropropagation armstatehydromet and ministry of agriculture yerevan, march 2012 rolot jeanlouis.
Multiple shoots were induced in vitro from nodal segments through forced. The purpose of stagei is to initiate axenic culture. Plant tissue culture technique has been used extensively to study the effect of. Micropropagation of mature pongamia pinnata pierre. Panigrahi is a fastgrowing, multipurpose tree of the humid tropic. Uses, benefits, cures, side effects, nutrients in pongamia pinnata. Multiple shoot bud induction and plant regeneration studies of. It is one of the few nfixing trees that produce oilseeds sangwan et al. Pongamia pinnata is drought resistant, semideciduous, nitrogen fixing leguminous tree. Department of biotechnology, gulbarga university, gulbarga585106, karnataka, india corresponding author email.
Introduction of disease free new cultivars is possible through micropropagation 6. It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. The plant extract contains flavonoids, carbohydrates, glycosides, international journal of chemtech. It is the fact that micropropagation is the only commercially viable method of clonal propagation of most of the horticultural crops. Biodiesel production from pongamia pinnata oil using synthesized iron nanocatalyst mookan rengasamy1, krishnasamy anbalagan2, sundaresan mohanraj2,velan pugalenthi2 1department of petrochemical technology, bharathidasan institute of technology, anna university, tiruchirappalli 620 024, tamil nadu, india. Micropropagation differs from all other conventional propagation methods in that aseptic conditions are essential to achieve success. Most of the tamil nadu physicians of indian system of traditional medicine ayurveda and siddha use pongamia pinnata to treat various kinds of diseases including diabetes mellitus5.
In our country there are more than 300 species of trees, which produce oil bearing trees. Literature survey of pongamia pinnata revealed its potential being like hepatoprotective and antioxidant 30. Lewis knudson, 1922 1st in vitro embryo germination orchid ie the invention of in vitro embryo culture. Aug 18, 2007 murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. It is found mainly in the native western ghats in india, northern australia, fiji and in some regions of eastern asia. These non edible oil seeds are also used for lightning purpose at night. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches 303 title. History of plant tissue culture propagation at cornell. Effects of pongamia pinnata on lipid peroxidation products and antioxidants in hyperammonemic rats with reference to circadian variations were evaluated by essa and subramanian 29. Murashige and skoog ms medium was used to establish the cultures of r. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. Detail study on the properties of pongamia pinnata. Definition of micropropagation clonal propagation in vitro micropropagation is the production of whole plant from small section of plant such as a stem tip, node, meristem, embryo, or even a seed.
Transesterification of crude oil of pongamia pinnata 2. Pierre a native indian biodiesel tree from cotyledonary node. Micropropagation of pongamia pinnata a sustainable biofuel plant species for semi arid climatic conditions vinod kumar patil and naik, g. With the increasing production of oil for biofuel, large amounts of oil cake are available for livestock feeding. Yeole2 1sanjivani college of pharmaceutical education and research, kopergaon, india. Derris indica bennet fabaceae pongam, indian beech. Micropropagation is labour intensive involving manual handling at 3 or 4 stages. Of the various culture media that have been assayed for micropropagation of. Pdf micropropagation of mature pongamia pinnata pierre. In case of dioecious species, where one of the sex is more desirable then under such circumstances plants of desired sex can be selectively multiplied by this technique.
Pierre is a medium sized glabrous tree popularly known as karanja in hindi, indian beech in english and pongam in tamil4. Micropropagation of mature pongamia pinnata pierre bioone. Pdf murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of. Micropropagation micropropagation of mature pongamia pinnata pierre k. Pongamia pinnata oil with conventional diesel with b10 and b20 proportions are furnished in the table3.
Pierre leguminaceae, subfamily papilionoideae is a medium sized tree commonly called as karanja that generally attains a height of about 1020 m and a trunk diameter of more than 50 cm pongamia. List of various diseases cured by pongamia pinnata. In vitro micropropagation of biofuel feedstock pongam pongamia. Transesterification of crude pongamia oil using koh. A simple and improved method for isolation of karanjin. Pair of meristematic masses mm developed from central meristem c. Micropropagation of orchids micropropagation is the process used to replicate plants such as orchids, the word micropropagation means simply propagation in miniature.
Knudson, l, 1922 nonsymbiotic germination of orchid seeds, botanical gazette lxxiii. Micropropagation of pongamia pinnata through enhanced. The rhs libraries can, for a fee, provide photocopies, by post if necessary. Effect of c y tokinin and aux in on shoot proliferation from cot yledonary nodes of pongamia pinnata grown on ms medium after 4 week. Biotechnology industry auxin auxins biodiesel fuels organic acids saturated fatty acids. Employing plant tissue culture techniques for micropropagation and further plant improvement of p. Micropropagation of mature pongamia pinnata pierre springerlink. Pdf the cotyledonary nodes were used as explant for developing a regeneration system for pongamia pinnata l. The process of micropropagation can be divided into four stages. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Micropropagation, micromorphological studies, and in vitro. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits.
Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. Curcuma longa is an indian spice derived from the rhizomes of the plant and has a long history of use in ayurvedic medicine as a treatment for inflammatory conditions. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. This stage begins with the excision of meristem tissue from an.
Pongamia pinnata is a native to many countries including india, malaysia, indonesia, taiwan, bangladesh, sri lanka and myanmar. Lewis knudson, 1922 1st in vitro embryo germination orchid ie the invention of in vitro embryo culture knudson, l, 1922 nonsymbiotic germination of orchid seeds, botanical gazette lxxiii. To multiply plants whose multiplication rate is very low. Karanja pongamia pinnata benefits, usage, ayurveda details. How pongamia pinnata is effective for various diseases is listed in repertory format. Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. Detail study on the properties of pongamia pinnata karanja.
The bud break was reported on ms medium supplemented with 1. The database and code is licensed under a creative commons attributionnoncommercialsharealike 3. With orchids it is the only way to reproduce the plants as. Pongamia pinnata, floral phenology, pod maturation, fruit colours, seed and seedling quality characters. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Culture media used in the initiation, shoot multiplication and rooting stages of the micropropagation of european chestnut via axillary shoots. Plant propagation by tissue culture is known as micropropagation. Chapter2 micropropagation and large scale culture of c. Creative commons attributionnoncommercialsharealike 3. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches.
Introduction to micropropagation cornell university. International journal of pharmtech research coden usa. It is useful in acne vulgaris, pimples with secretions. It is done under sterile conditions in a laboratory using plant seed or tissue. A tissue culture protocol was developed for an important medicinal plant rungia pectinata l. Gavino rotor, 1949 1st in vitro vegetative propagation.
It is a source of biodiesel and is used in ayurveda for external application to shrink pile mass, to heal wounds and abscesses quickly. Micropropagation is the only viable method of multiplying genetically modified cells or cells after protoplast fusion. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. Enhancing rate of multiplication shoot multiplication short cycle 26 weeks small size propagules storage on large scale, easily transported. Preparation of biodiesel from crude oil of pongamia pinnata. Research article physico chemical analysis of pongamia. Micropropagation is the growing of plants from meristematic tissue or somatic cells of superior plants on nutrient suitable media under controlled aseptic physical conditions.
Axillary buds, biodiesel plant, leaf, micropropagation, pongamia, plant growth regulators, plant tissue culture. Micropropagation enables growers to increase the production of plants that normally propagate very slowly such as narcissus and other bulbous crops. Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. Research article physico chemical analysis of pongamia pinnata.
This is particularly valuable in the case of difficultto. To 10 g of crude pongamia oil, a known amount of catalyst koh, 0. The main characteristic of this technique is rapid clonal multiplication which results in the production of a large number of uniform plants from a limited number of mother stock plants. Murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Pongamia pinnata grows much faster than any other bush and fixes co 2 as its stem and branches, thus reducing co 2 from atmosphere and offer biomass. It grows about 1520 meters in height with a large canopy which spreads equally wide. Vegetative propagation of sterile hybrids can be used as parent plants for seed production. Methods of micropropagation methods used in micro propagation are based on the production of sterile tissue,stimulation of regeneration,rapid growth of the young plantlets, rooting of plantlets and their weaning onto normal soil conditions. It is the truetotype propagation of a genotype that comprises many stepsstock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation, and growing on of liners and is usually. It is also used to provide a sufficient number of plantlets for planting from a stock plant. Pongamia pinnata, karanjin, karanja oil, medicinal oil, hplc.
Oct 16, 2007 a complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Sanskrit name karanja taila kannada name honge enne. Millettia pinnata, also known as pongamia pinnata or pongamia glabra, is common throughout asia and thus has many different names in different languages, many of which have come to be used in english to describe the seed oil derived from m. Multiple shoot bud induction and plant regeneration. Pdf micropropagation of karanj pongamia pinnata pierre. The collection and extraction of oil is carried out by indian biodiesel corporation, baramati.
In plants, the dna of interest is transferred into the new plant by using agrobacterium tumefaciens, a bacterium that can infect plant tissues and incorporate part of. Pongamia indian beech is a genus having one species pongamia pinnata l. General physical properties of pongamia pinnata oil blends b10, b20 and diesel parameters pongamia pinnata diesel b20b10 ph 3. Micropropagation is a combination of the arts and sciences of plant multiplication in vitroand plant acclimitization.
Pongamia oil is derived from the seeds of the millettia pinnata tree, which is native to tropical and temperate asia. Useful tropical plants database 2014 by ken fern, web interface by ajna fern with help from richard morris. A piece of plant tissue called an explant is a cut from the plant, b disinfested removal of surface contaminants, and c. Your use of this pdf, the bioone complete website, and all posted and associated.
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